用滤波器提高共焦系统纵向分辨率的实验研究

Improvement of Axial Resolution in Confocal Microscopy by Filters

针对采用有限大小探测器的共焦显微系统,利用两区相位型滤波器提高系统的纵向分辨率。在实验上,采用1透射式液晶空间光调制器实现了对共焦显微系统照明光场位相的动态调制,测量了探测针孔直径分别为5μm、10μm和15μm时,共焦显微系统纵向分辨率随滤波器参数的变化规律。实验结果说明,在探测器为有限大小的情况下,利用该滤波器可以使共焦显微系统的纵向分辨率提高17.8%。另外,从实验结果还可以看出,滤波器的性能受到探测针孔尺寸的影响。

For a confocal microscope with a finite-sized detector, the improvement in axial resolution by a phase-only filter with two zones was experimentally investigated. The illumination path of the confocal system was dynamically modulated by a liquid-crystal spatial light modulator operating in a transmission-only mode. The variations of axial resolution with filter parameters were measured, when pin-hole diameter were 5 μm, 10 μm and 15 μm, respectively. The experimental results show that the axial resolution of confocal microscope can be improved by 17.8%, in the case of a finite-sized detector. It can be concluded that filter performance is influenced by pinhole size.