自制与商业化抗人精液蛋白P_(30)金标试剂条的法医学应用比较

The forensic availability of home-made and commercial strips of gold-labeled anti-human seminal protein of P_(30)

目的 检测比较自制和商业销售的抗人精液蛋白P30 金标试剂条 ,探讨它们法医学应用的可靠性。方法 以人精液和人前列腺特异蛋白检测效价及灵敏度 ,以人阴道分泌物、人血清、人唾液和人初乳检测特异性 ,以保存不同年限的陈旧人精斑和斑痕类检材测定检验实际检材的能力。结果 自制抗人精液蛋白P30 金标试剂条效价及灵敏度与商业销售的试剂条中较好的基本相当 ,特异性则相当或好于商业试剂条 ,对非特异性测定均为阴性 ,而商业化试剂条则多有非特异性反应。结论 使用商业金标试剂条 ,要确定其是否合乎法医学检验标准与要求。

Objective To compare home-made and commercial gold-labeled strips of anti-human seminal protein of P_(30) to explore and define their availability on forensic examination. Method Titers and sensitivities were detected with the antigen of a mixture of seminal fluid from 6 people and the purified human prostate specific antigen, whereas specificities were with feminine vaginal secretions, human sera, human saliva and human colostrums, and the power identifying human seminal stains was established with old human seminal stains and blind-drawn material evidences. Results For the titers and sensitivities the home-made strip of gold-labeled anti-human seminal protein of P_(30) was equal to the better ones of those commercial, but on specificities the home-made one is at least equal or even better than the commercial ones, having no reactions against the tested substances used for the establishment of specificity, however the commercial ones were most of all having non-specific reactions. Conclusion It is very important to select the strips adaptable to the requirements of forensic examination, so there exists a necessity to define their forensic availability for commercial strips before use.