摘要
本文采用组培愈伤组织接种法研究了不同抗病类型的杨树品种受溃疡病菌(Dothiorellagregaria Sacc.)侵染后苯丙氨酸解氨酶活性以及绿原酸和木质素含量的变化与抗病性的关系。纳果发现,受病菌侵染后各品种的苯丙氨酸解氮酶活性都有一个明显消长过程,但抗病品种的酶活性升高的幅度大(为感病品种的2倍),持续的时间也长;伴随酶活性的变化,各品种的绿原酸含量也发生明显变化,与感病品种相比,抗病品种的绿原酸不仅出现得早,而且积累的量也大(为感病品种的三倍);木质素的增量也与品种的抗病性呈正相关。上述结果表明,杨树对溃疡病的抗性与苯丙氨酸解氨酶的活性密切相关,该酶在抗病中的作用是通过其控制的代谢产物得以以表达。
The callus of poplar varieties with different resistance to poplar cankerwere inoculated with the pathogen,Dothiorella gregaria.Twelve hours afterinoculation,all varieties appeared to increase in enzymatic activity ofphenylalanine ammonia lyase(PAL),yet the PAL activity of the resistantvarieties was twice higher and longer(for 48 hours)than that of the suscep-tible ones,in which the enzymatic activity decreased rapidly and came toits normal level after 36 hours.Accompanied with the increase of PAL acti-vity,chlorogenic acid in poplar trees accumulated rapidly.However theaccumulation of chlorogenic acid in the resistant varieties was not onlyfaster,but also greater and the acid amount was three times of the suscep-tible ones.It was also found that the amount of lignin was positively corre-lated with the disease resistance of the tested poplar varieties.The resultsshowed that there was a direct interrelationship between the activity of PALas well as the amount of chlorogenic acid and lignin and the resistance ofpoplar to poplar canker.
出处
《植物病理学报》
CAS
CSCD
北大核心
1992年第2期185-188,共4页
Acta Phytopathologica Sinica
