胃黏膜对慢性乙醇刺激适应性机制的初步探讨

Mechanism of adaptive reaction of gastric mucosa caused by chronic stimulation of ethanol

目的 :探讨胃黏膜对慢性乙醇刺激所发生的适应性反应机制。方法 :用低浓度乙醇持续给大鼠灌胃 ,在 4周末、8周末和 12周末处死动物 ,观察胃黏膜的组织损伤情况 ;用免疫组织化学方法测定不同时期胃黏膜中转化生长因子α(TGF α)的表达强度。结果 :随着乙醇的持续刺激模型组胃黏膜肉眼可见自糜烂到小溃疡形成 ,镜下由充血、水肿到腺体萎缩 ;炎性细胞浸润的种类也由早期的中性粒细胞为主到多种炎性细胞混合 ,3个月后主要是淋巴细胞和浆细胞 ;胃黏膜组织内TGF α也随乙醇刺激时间的延长表达逐渐增强。结论 :长期持续乙醇刺激可导致胃黏膜腺体萎缩 ,同时炎性细胞浸润的种类随时间的推移有明显不同 ,这种适应性反应可能与TGF -α的表达增强有关。

Objective: To study the adaptive reaction of gastric mucosa caused by chronic stimulation of ethanol. Methods: Rats received ethanol orally 3 times per day and were killed at the end of the 4th, 8th, 12th weeks. The changes of gastric mucosa and expression of transforming growth factor (TGF-α)were observed during different stages. Results: With the successive stimulation of ethanol, the gastric mucosa showed erosion to small ulcer observed by nake eyes and hyperemia adema to glandular atrophy observed with microscope. In early stage, neutrophil was dominant and 3 months later, lymphocyte and plasma cell infiltrated predominantly. The expression of TGF-α was increased with successive ethanol stimulation. Conclusion: Long term of chronic stimulation of ethanol induce glandular atrophy of gastric mucosa. Different kinds of inflammatory cells infiltrated at different stages. The adaptive reaction of gastric mucosa may be associated with the up-regulation of TGF-α expression.