糖液预处理对提高玉米花培诱导率的研究

Study on Increasing Anther Culture Induction Frequency of Soaking Maize Anther with Sucrose

对不同材料用蔗糖溶液预处理花药,使之产生质壁分离,再接种于不同蔗糖浓度的培养基上。探讨了玉米花药胚状体(愈伤组织)产生情况,指出胚状体(愈伤组织)与基因型关系很大,用25%浓度蔗糖预处理花药6～7min及培养基中加15%浓度蔗糖诱导频率最高。

The paper discussed the effects on soaking anther of different materials and liquor of different concentration first to make cytoplasm break away from cell wall,then inoculating the anther in medium of different concentration sucrose.Results showed that it is different about the embryo(callus)induction frequency of different materials.The induction frequency of soaking anther with media of25%sucrose for6～7minute and inoculating15%sucrose medium is the highest.