白藜芦醇对鼻咽癌细胞株CNE-2Z增殖和凋亡的影响

Effects of resveratrol on proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE-2Z

目的 探讨白藜芦醇 (resveratrol,Res)对鼻咽癌细胞株 CNE- 2 Z增殖和凋亡的影响。方法 采用 MTT法检测 IC50 值 ,流式细胞术、Hoechst 332 5 8/PI荧光染色和 DNA琼脂糖凝胶电泳分析细胞凋亡。结果 不同浓度Res分别处理细胞 2 4 ,4 8和 72 h,其 IC50 值分别为 (10 9.2± 7.5 ) ,(83.6± 6 .0 ) ,(5 4 .3± 2 .8) μmol/L,各 IC50 值间比较差异显著 (P<0 .0 1)。2 5 ,5 0 ,10 0和 2 0 0 μm ol/L Res分别处理细胞 2 4 h后 ,5 0 ,10 0 ,2 0 0 μmol/L Res处理组经流式细胞术和荧光染色检测出的细胞凋亡率均明显高于对照组 (P<0 .0 1) ,荧光染色可见典型的凋亡形态学改变 ,10 0 ,2 0 0 μmol/L Res处理组可见明显的 DNA梯带。结论 Res可通过诱导 CNE- 2 Z细胞株凋亡而抑制其增殖。

Object To investigate the effects of resveratrol (Res) on the proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE-2Z. Methods IC 50 values were detected by MTT assay. The apoptosis was analyzed by flow cytometry (FCM), Hoechst 33258/PI fluorescence staining, and DNA agarose gel electrophoresis. Results After CNE-2Z cells were treated with different concentrations of Res for 24, 48, and 72 h, their IC 50 values were (109.2±7.5), (83.6±6.0), and (54.3±2.8) μmol/L respectively (P<0.01). After CNE-2Z cells were treated with 25, 50, 100, and 200 μmol/L Res for 24 h, the apoptotic rates by both flow cytometry and fluorescence staining at 50, 100 and 200 μmol/L Res-treated groups were significantly higher than those in control groups (P<0.01). The typical morphological changes in apoptosis were found by fluorescence staining. The results of DNA agarose gel electrophoresis showed that DNA ladder appeared obviously at 100 and 200 μmol/L Res-treated groups. Conclusion Res can inhibit the proliferation of cell line CNE-2Z through the induction of apoptosis.