内源性睾酮对损伤的血管内皮细胞功能和结构的影响

Effects of endogenous testosterone on vascular endothelial cell function and structure in rabbits of balloon injury

目的 观察内源性睾酮对球囊损伤的血管内皮功能和结构的影响。方法 2 0只雄性家兔随机等分为去势组和对照组 ,去势组行去势术 (切除双侧睾丸和附睾 ) ,对照组行相应假手术 ;均于术后 2周行右侧髂动脉内皮剥脱术。观察去势 (或相应假手术 )前和脱内皮术后 1周、2周血浆一氧化氮 (NO)、内皮素 1(ET 1)水平的变化。脱内皮术后 2周将动物处死 ,取损伤血管段 ,应用电镜和HE染色观察血管病理形态的变化。结果 两组间同时间点相比 ,血浆NO、ET 1水平变化差异均无显著性 ,但脱内皮术后 2周去势组血浆NO、ET 1水平可恢复至去势前水平 ,去势组比对照组 ,NO为 (12 5±2 2 ) μmol/Lvs (130± 18) μmol/L(P >0 0 5 ) ,ET 1为 (81± 8)ng/Lvs (75± 2 2 )ng/L(P >0 0 5 )。而对照组与实验前水平仍存在统计学差异 ,NO为 (98± 19) μmol/Lvs (14 1± 2 0 ) μmol/L(P <0 0 5 ) ,ET 1为(91± 9)ng/Lvs (71± 12 )ng/L(P <0 0 5 )。电镜下两组血管内膜均几乎完全被新生内皮覆盖 ,再生血管内皮细胞 (VEC)胞体肥大 ,轮廓模糊 ;光镜观察两组损伤血管的内膜面积和内膜 /中膜面积比值差异无显著性。结论 内源性睾酮水平短时低于生理水平时对VEC再生、新生内膜形成无明显影响 ,但有改善损伤的VEC功能的倾向。

Objective To study the influences of endogenous testosterone on vascular endothelial cell function and structure in rabbits of balloon injury. Methods Twenty male rabbits were randomly divided into two groups: castrated group and control group.The former group animals were castrated and the later group animals received sham operation. All animals were performed with deendothelializing balloon injury in the right iliac artery. Nitric oxide (NO) and endothelin-1 (ET-1) were measured at different intervals during the experiment. After 2 weaks of endothelial denudation all animals were killed, and endothelin structure and morphological changes were observed with electron microscope and HE staining. Results There weren′t siginificant differences of plasma NO and ET-1 levels between the two groups, but plasma NO and ET-1 levels of the castrated group 2 weaks after endothelial denudation recovered to the levels before castration, while they didn′t in the control group. The extent of reendothelialization and intimal area of the harvested segments were similar between the two groups. Conclusion The decrease of endogenous testosterone level did not affect endothelial cell structure, but tended to protect function of injured endothelium.