运用彗星试验检测麝香酮对苯并(a)芘致DNA损伤的影响

A Study of Effect of Musk Ketone on Benzo(a) pyrene-induced DNA Damage by Comet Assay

[目的]研究麝香酮在体内试验条件下 ,对苯并(a)芘致小鼠肝和肺细胞核DNA损伤的影响。[方法]SENCAR雄性成年小鼠灌胃 ,分别单独给予苯并(a)芘125、250、500mg/(kg·bw·d)和用麝香酮250、500mg/(kg·bw·d)预处理后再给予苯并(a)芘125mg/(kg·bw·d) ,处死动物后分离小鼠肝和肺组织细胞核做彗星测试 ,采用CCD成像分析系统分析彗星 ,取尾相 (olivertailmoment,OTM)值判断DNA损伤强度。[结果]苯并(a)芘各浓度组均出现小鼠肝和肺细胞OTM值的明显增加 ,高浓度麝香酮[500mg/(kg·bw·d)]可引起小鼠肝细胞OTM值的轻度增加。用高浓度麝香酮预处理小鼠后再给予苯并(a)芘 ,所观察到的肝、肺的DNA损伤 (肝OTM值为7.6、肺为11)较之苯并(a)芘单独染毒引起的DNA损伤(肝OTM值为5.1、肺为6.9)增加更明显。[结论]麝香酮能明显增强苯并(a)

Objective To study the effect of musk ketone(MK)on benzo[a]pyrene(B[a]P)-induced nuclear DNA damage of liver and lung in mice using comet assay.[Methods]Male SENCAR mice were dosed by oral gavage with B[a]P(125,250,500mg/kg b.w.)alone once or B[a]P(125mg/kg b.w.)once after pretreatment with MK(250,500mg/kg b.w.)for two days.Nuclei from liver and lung cells of mice were prepared for comet assay.The“comets”of102randomly selected nuclei per organ per mouse were measured using a CCD image analysis system(IAS,Optilas comet system).The“oliver tail moment(OTM)”of each core was used as indicator of DNA damage.[Results]The significant increased OTMwas shown in liver cells and lung cells of mice treated alone with B [a]P at all of dosage.MK induced a mild increase of OTMin liver cells of mice exposed to MK at higher concentration(500mg/kg b.w.).The significant increased OTMwas observed in liver and lung cells of mice when animals were pretreared with higher concenˉtration MKand then B[a]P(OTM=7.6in liver cells and OTM=11in lung cells)in comparison with B[a]P alone treatment(OTM=5.1in liver cells and OTM=6.9in lung cells).[Conclusion]MK possess co-effect of DNA damage towards B[a]P.