天麻对铝染毒大鼠大脑皮质氨基酸递质的影响

Effects of Gastrodia Elata on the Alteration of Amino Acid in the Brain Cortex Induced by Aluminum in Rats

[目的]探讨天麻对铝染毒大鼠大脑皮质氨基酸类神经递质的影响。[方法]将SD大鼠36只在动物室适应性喂养1周后 ,分成6组 ,腹腔注射生理盐水或AlCl3 溶液0.2ml/d ,60d。每连续注射3d间歇1d ,60d内共计注射45次。在间歇天肌肉注射青链霉素以抗感染。整个染毒期间 ,动物室以自然节律采光 ,温度18～23℃ ,湿度40%～60 % ,清洁 ,安静。所有动物饲以普通饲料 ,自由饮水和进食 ,加天麻组 ,以0.4g/kg体重的剂量在饮水中加入天麻。每周定时称体重一次 ,调整Al的浓度和饮水中天麻的浓度。染毒期结束后 ,将大鼠断头取脑 ,在冰冷的生理盐水中漂洗、去脑膜、用滤纸拭干。取大脑皮质0.2g(湿重 ) ,加浓硝酸1.6ml,高氯酸0.4ml,消化后 ,用去离子水定容至1.5ml,用石墨炉原子吸收光谱法测定脑铝含量。取左侧大脑半球额叶皮质0.1g,用脑组织处理液以1:50( g:ml)作成匀浆 ,用HLPC法测定氨基酸含量。[结果]与生理盐水组相比 ,Al3 +5mg/kg组和Al3+10mg/kg组脑铝含量均显著升高 (P<0.01) ;生理盐水 +天麻组与生理盐水组相比、Al3+5mg/kg+天麻组与Al3+5mg/kg组相比及Al3 +10mg/kg+天麻组与Al3 +10mg/kg组相比 ,大鼠大脑皮质铝含量差异未见统计学意义(P>0.05)。大脑皮质氨基酸含量结果显示 ,与生理盐水组相比 ,Al3+ 5mg/kg组谷氨酸

Objective To investigate the effect of Gastrodia elata(G.E.)on the content of amino acid in the brain cortex of rats exposed to aluminum.[Methods]36SD rats,adult and healthy,were divided into6groups at random:①normal saline,②Al 3+ 5mg/kg,③Al 3+ 10mg/kg,④normal saline+G.E.,⑤Al 3+ 5mg/kg+G.E.and⑥Al 3+ 10mg/kg+G.E..The rats were given AlCl 3 by intraperitoneal injection at a dose of0.2ml/d for60d.G.E.was administered through drinking water at a dose of0.4g/kg body weight.Glu and GABA were determined by HPLC.[Results]The result showed that compared with the normal saline group,the content of aˉluminumin brain cortexincreased significantly in the group of Al 3+ 5mg/kg and Al 3+ 10mg/kg(P<0.01);compared with the group of Al 3+ 5mg/kg andAl 3+ 10mg/kg correspondently,the content of aluminumin brain cortex is not significantly different fromthat in the group of Al 3+ 5mg/kg+G.E.and Al 3+ 10mg/kg+G.E.respectively.Compared with the normal saline group,the content of Glu deˉcreased(P<0.05)and the content of GABA increased(P<0.01)significantly in the Al 3+ 5mg/kg and Al 3+ 10mg/kg groups;comˉpared with the Al 3+ 10mg/kg group,the content of Glu increased(P<0.05)and the content of GABA decreased(P<0.01)obviously in the Al 3+ 10mg/kg+G.E.group.[Conclusion]Aluminumexposure can induce the disorder of amino acid systemin the brain cortex of rats and the effect that G.E.affects amino acid neuro transmitter system maybe is not due to reducing brain cortex aluminum.The interaction between aluminum and G.E.is unknown.