人参皂甙Rg1对体外磷酸二酯酶5活性的影响

The investigation of ginkgo glycoside on PDE5

目的 :观察人参皂甙Rg1对磷酸二酯酶 5 (PDE5 )酶活性的影响。 方法 :应用12 5I放射免疫法 ,测定不同浓度的人参皂甙Rg1对PDE5活性的影响 ,并以西地那非作为阳性对照。 结果 :人参皂甙Rg1对PDE5有抑制作用 ,从而阻断cGMP的水解 ,能提高cGMP水平。结论 :人参皂甙Rg1能有效抑制PDE5活性 ,其半数有效浓度IC50 为 7 2 4nmoI/L ;西地那非IC50 为 3 4 2nmol/L。

Objective: To investigate the effect of ginkgo glycoside on PDE5. Methods: Use 125I RIA kit to measure the effect of different concentration of ginkgo glycoside on cGMP--the substrate of PDE5 with sildenafil as a control. Results: The ginkgo glycoside could inhibit the activity of PDE5 and elevate the level of cGMP. Conclusion: The ginkgo glycoside Rg1 could inhibit the activity of PDE5 effectively and IC 50 of ginkgo glycoside was 7.24 nmol/L, while IC 50 of sildenafil was 3.42 nmol/L.