肉毒杆菌毒素对兔颞下颌关节骨关节病动物模型的关节组织病理学影响

Histopathological effects of botulismotoxin to temporomandibular joint osteoarthrosis of animal models

目的:观察颞下颌关节骨关节病动物模型应用肉毒杆菌毒素后关节组织学改变,证明它对该病的治疗作用。方法:应用胶原酶注射法制备30例兔TMJOA的动物模型,分成对照组与实验组,实验组双侧颞肌、嚼肌内注射肉毒杆菌毒素,将实验组与对照组在4周末、8周末、12周末各处死5只。取出关节盘及髁状突,分别观察其在光镜及扫描电镜下的病理变化。结果:4周末时,对照组动物出现了明显的骨关节病变化,关节盘血管增生明显和软骨细胞增生活跃,实验组病变较轻,第8周末时,对照组病变更典型,出现软骨裂隙和凝胶样物质剥脱,实验组出现病变修复;12周末时,对照组出现严重的病理改变,软骨剥脱,骨赘形成,而实验组病变修复接近正常。结论:肉毒杆菌毒素可以抑制TMJOA的发展,促进关节的自身修复,对TMJOA有显著的治疗作用。

Objective:To study the effects of the botulismotoxin in treating temporomandibular joint osteoarthrosis (TMJOA) accoding to observing histopathological change of botulismotoxin to rabbit TMJOA. Method: TMJOA models in 30 adult rabbits were made by injecting with collagenase and divided randomly into experimental and control groups.In experimental group, botulismotoxin was injected in bilateral masseter muscles (MM) and temporalis muscles (TM). Five rabbits of each group were sacrificed at the end of the 4th,8th,12th week respectively, then involved articular discs and condyloid processes were removed to make sections and observed under light microscope and scanning electron microscope.Results:At the end of the 4th week,under microscope,in control samples apparent osteoarthrosis changes were showed--apparent and active vessel hyperplasia of arthal disc and cartilage cells,but slight histopathological change in experimental samples.At the end of the 8th week,more typical changes were found in control samples-appearance of cartilage crevice and exfloliation of gelatinoid substance,but origination of repairment in experimental samples. At the end of the 12th week, seriously pathological changes were found-cartilage exfloliation and osteoneoplasms in control samples,but almost complete repairment in experimental samples. Conclusion: Botulismotoxion was significantly efficient to treat TMJOA by inhibiting the progress of TMJOA and promoting the self-repairment of TMJOA.