摘要
三七(Panax notoginseng)细胞发酵培养的皂甙含量为干重的11.21%,皂甙产率为1513.3mg·L^(-1),细胞培养物产率为每月13.58g干重·L^(-1),均比悬浮培养高。接种量的增加能明显地提高生长速率、皂甙产率以及细胞培养物产率。通气速率0.8vvm较适合于三七细胞的发酵培养。在发酵培养过程中pH值由5.8逐渐降至3.92,没有出现回升。
The saponin content of Panax notoginseng cell cultures was 11.14% of dry weight,the saponin yield was 1513.3mg·L^(-1),and yield of cell cultures was 13.58g dry wt·L^(-1) per month in fermentation culture,which were all better than those obtained from in suspension culture.Increasing inoculum quantity could obviously increase growth rate,saponin content and yield of cell cultures.An aeration rate of 0.8vvm was optimal for fermentation culture of the cells.The pH value of the culture broth went down from 5.80 to 3.92 gradually and never returned in ferme- ntation culture of P.notoginseng cells.
关键词
三七
发酵培养
Panax notoginseng
Fermentation culture
Saponin
Growth rate
