摘要
以水稻(Oryza sativa L.)成熟种子为材料,基本培养基MS和B5,诱导产生体细胞胚状体。实验结果表明:水稻不同品种与体细胞胚状体的产生有一定的相关性;诱导期2,4-D浓度对胚伏体形成十分重要。在适宜于诱导的水稻品种上,胚状体形成的不同阶段对诱导因子的要求十分严格。KT,zeatin,BAP,ABA,渗透压等因子均影响胚状体的形成。胚性愈伤组织的分层培养结果表明:胚状体来源于愈伤组织表层的胚性细胞。诱导期愈伤组织无胚状体形成。在液体分化培养基Ⅰ上胚状体产生频率最高达70％以上。
On MS and B5 basic media the mature seed of rice (Oryza sativa L.) was induced to produce somatic embryoid. The results showed that the rate of occurrence of somatic embry- oid varied with the variety of rice and the concentration of 2,4-D was important to the occur- rence of somatic embryoid. For those varieties of rice suitable for induction, the induction factors such as KT, zeatin, BAP, ABA, osmotic pressure and so forth were stringently requi- red at different stages of somatic embryoid development, and they also influenced the forma- tion of somatic embryoid. The results of various layered cultures of embryonic calli proved that the somatic embryoid was derived from the surface layer callus containing embryonic cells. At the induction stage no somatic embryoid was formed in callus. The rate of somatic embryoid formation was over seventy per cent on liquid differentiation medium I.
关键词
体细胞胚状体
悬浮培养
水稻
Mature seed of rice
Somatic embryoid
Suspension culture
Induction factors
