摘要
大豆胚状体可以直接从未成熟的子叶表皮及表皮下面1—3层细胞发生。这些细胞经过脱分化后,首先形成细胞质浓厚、核大的胚的发生细胞,胚发生细胞再分裂形成胚性细胞团,胚性细胞团再继续分裂形成胚状体。胚状体的发育过程和合子胚一样,经过球形、心形,鱼雷期和子叶期等诸阶段发育成小植株。此外,在诱导胚状体发生过程中,还观察到另一值得注意的现象:在未成熟胚的子叶表皮下面1至较深处的数层细胞,也转变成分生状细胞团,这些分生状细胞团呈不规则状,从其起源看,可称它们为内生“胚状体”,这些内生“胚状体”培养至20天,即停止生长发育。
This paper deals with a histological observation of somatic embryogenesis of soybeam cultured in vitro,an extension to our previous on“Plant regeneration from immature embyro culture of soybean via somatic embryogenesis”.The results are presented as follows:The em- bryonic cell mass appeared at first by initiating from the cells of epidermis and 1—3 layers of subepidermis of the immature cotyledon which underwent de-differentiation.After that, the embryoic cell mass continued to divide and developed further into embryoids.Later on, the embryoids developed into plantlets throughout globular,heart-shaped,torpedo-shaped and cotyledon stages.Apart from this,during process of embryoid induction,another noticeable feature was that the embryonic cell might also be produced in the deeper layers beneath the epidermis of cotyledon.They looked irregular and developed into globular or heart-shaped cell mass.Judging from their origin,they might be“esdo-embryoids”.Whether these“endo- embryoids”could be further developed is a question that warrants further studies.
关键词
体细胞胚发生
组织学
大豆
Soybean
Histological studies
Somatic embryogenesis
