自体盆神经节海绵体内移植对大鼠阴茎nNOS的影响

Homotransplanted Major Pelvic Ganglion:Effects on nNOS in the Corpous Cavernosurm of Adult Rats

目的 研究双侧海绵体神经离断后 ,自体盆神经节海绵体内移植对大鼠阴茎神经型一氧化氮合成酶(nNOS)的影响。方法 36只雄性SD大鼠 (3～ 6个月 ,30 0～ 4 0 0克 )随机分为 3组 :实验组 (双侧海绵体神经离断 +左盆神经节左侧阴茎脚海绵体内移植 )、异常对照组 (双侧海绵体神经离断 )、正常对照组 (假手术组 )。 6周及 12周后 ,免疫组化SP法检测各组阴茎中段及实验组左侧阴茎脚组织中nNOS的表达。结果 实验组阴茎中段nNOS的表达与异常对照组并无显著差异 (P >0 .0 5 ) ,与正常组有显著性差异 (P <0 .0 5 ) ,但神经节移植区域 (左阴茎脚 )可见显著增多的nNOS阳性表达的神经细胞及纤维 (P <0 .0 5 )。结论 盆神经节海绵体内移植后远端阴茎组织内nNOS无明显变化 ,但移植区域nNOS表达显著增加 ,盆神经节已存活。

Objective The purpose of this paper is to investigate effects of homotransplanted major pelvic ganglion on nNOS in the corpous cavernosurm of adult rats after bilateral cavernous nerve injury. Methods 36 male Sprague-Dawley rats(3-4m old and 300～400g/each) were randomly divided into three groups:(a) bilateral cavernous nerve transection and transplantation of left major pelvic ganglion into left crus(experimental group); (b)bilateral cavernous nerve transection and (c) sham-operated controls. At the end of 6th and 12th week all rats were sacrificed to detect the expression of nNOS in penis of all groups and ganglion implanted area(the left crus) of experimental group by SP method. Results There was no significant difference in expression of nNOS in penis between experimental group and bilateral cavernous nerve transection group(P >0.05), both were lower than that of sham-operated(P <0.05). Much more nNOS containing nerve cell bodies and fibers was observed in ganglion implanted area than in non-implanted area(P<0.05). Conclusion The homotransplanted major pelvic ganglion may survive in the corpous cavernosurm and express nNOS, though it doesn't improve the nNOS expression of whole cavernous tissue.