摘要
目的 利用高脂饮食建立兔动脉粥样硬化模型 ,观察Lox 1在动脉组织中的表达和氯沙坦对Lox 1及斑块的影响。方法 将雄性新西兰白兔按体重随机分为 3组 :对照组 6只 ;高胆固醇组 8只 ;氯沙坦组 8只。检测 0、10周血脂水平 ;计算主动脉AS斑块面积 ;RT PCR法检测动脉组织中Lox 1mRNA表达的水平。结果 与正常对照组比较 ,高脂饮食增加血脂水平、斑块面积和Lox 1(mRNA)表达 (均P <0 .0 1)。应用氯沙坦后血脂较高胆固醇组无明显变化 (P >0 .0 5 ) ,而斑块面积、Lox 1表达不及高胆固醇组明显 (P <0 .0 1)。结论 Lox 1与动脉粥样硬化的发生、发展有着紧密的联系 ,高脂饮食促进Lox 1表达。氯沙坦可抑制Lox 1表达 ,具有抗AS的作用。
Objective To examine the expression of Lox-1 in aorta of cholesterol-fed rabbits and the effects of Losartan on etherosclerotic lesion and Lox-1. Methods 22 rabbits were divided into 3 groups: normal diet group, high-cholesterol group and Losartan group(25mg/kg·d). The blood samples were collected for cholesterol level at week 0 and week 10. LOX-1 expression in aortas was examined by semiquantitative RT-PCR. Results High-cholesterol diet induced hyperchol-esterolemia, formation of aortic lesion and increase in LOX-1 mRNA expression in the endothelium and neointima. Treatment with losartan decreased the enhanced LOX-1 expression. But the level of cholesterol did not alter significantly. Conclusion High-cholesterol diet induces the upregulation of LOX-1 expression in neointima of aortas of rabbits. Treatment with losartan, an AT1 blocker, markedly decreases this enhanced LOX-1 expression. Losartan has antiatherogenic effect.
出处
《医学新知》
CAS
2004年第1期40-43,共4页
New Medicine
