摘要
用双向聚丙烯酰胺凝胶电泳技术,将光周期敏感核不育水稻“农垦58S”和其对照品种“农垦58”苗期及育性转换光周期敏感期的叶绿体蛋白质分离为大约90个蛋白质点。“农垦58S”的叶绿体内有一个45kD(pI_(6.7))和一个61kD(pI_(6.0))的特异性蛋白质点,而“农垦58”没有。“农垦58S”的另一个61kD(pI_(6.2))蛋白质点的含量明显高于“农垦58”。不同光周期(长日照和短日照)处理不影响光敏感期的这种叶绿体蛋白质的差异。
The chloroplast proteins of a male-sterile mutant Nongken 58S,namely“Hubei Photo- period-sensitive Genic Male-sterile Rice”,which is male sterile under long day(LD)cycles and fertile under short day(SD)cycles,and its original cuhivar Nongken 58(Oryza sativa L. subsp.japonica,Nongken 58)at seedling stage and photoperiod sensitive stage of fertility transition could be resolved into at least 20 major and more than 70 minor protein components on two-dimensional gel electrophoresis,with molecular weights ranging from 10kD to 67kD and isoelc points(pl)from 4.3 to 8.5.The mutant Nongken 58S had twospecific proteins with molecular weight of 45kD(pI_(6.7))and 61 kD(pi_(60.))as compared with Nongken 58.Another 61 kD(pI_(62))protein was more in Nongken 58S than in Nongken 58. The existance of these proteins was not influenced by SD or LD treatment.The results showed difference in patterns of gene expression between Nongken 58S and Nongken 58.A possible function of these proteins in relation to regulation of sterility was discussed.
基金
国家高技术研究发展计划资助课题
关键词
叶绿体
水稻
雄性不育
特异性蛋白
Photoperiod-Sensitive Genic Male-Sterile Rice
Chloroplast
Specific proteins
