白芷原生质体培养及植株再生

PROTOPLAST CULTURE AND PLANT REGENERATION OF ANGELICA DAHURICA

白芷(Angelica dahurica)茎节诱导产生的愈伤组织在含1mg/L 2,4-D 的 MS 培养基上继代培养,半年左右,当白色松软的愈伤组织内部形成较多胞质稠密的细胞团时,分离的原生质体才有再生植株的能力。原生质体衍生的细胞团可继续发育成胚或长大成愈伤组织,二者比例受培养基中激素及渗透压的影响。无论直接形成的胚或后来从愈伤组织分化出的胚都在合适的条件下发育成完整植株。

White and soft calli were induced from +~he stemnodes of Angelica dahurica on MS medium containing lmg/L 2,4-D, and subcultured on the same medium with decreased concentration of the hormone for about half a year, until quite a number of embryogenic cell clusters were produ- ced in calli. Protoplasts prepared only from this kind of callus were regenerable. The proto- plasts-derived colonies were able to develop into embryos directly or to grow continously into calli as affected by the hormone and, in particular, by osmotic pressure in the culture medium. The embryos either formed directly or via callus stage were all capable of regenerating complete plants under proper culture conditions.