黄芪对高能冲击波致肾损伤保护性作用的实验研究

Experimental study of the protective effects of Astragalus membranaceus (huangqi) on renal damage induced by ESWL

目的 探讨黄芪对高能冲击波致肾损伤的保护作用及可能机理。 方法 将 4 5只家兔随机分为 3组 :黄芪组、异搏定对照组和生理盐水假治疗组 ,行高能冲击波前 3d分别静脉注射黄芪注射液 2 .4g/kg、异搏定 0 .4mg/kg、生理盐水 2 0ml,观察家兔血、肾组织匀浆中损伤相关因子 (ET 1、MDA)和损伤保护相关因子 (SOD)及肾组织细胞情况。 结果 冲击后异搏定组和黄芪组血和组织匀浆中ET 1、MDA升高幅度均显著低于假治疗组 (P均 <0 .0 5 ) ,且 1周恢复正常水平 ;黄芪组血和组织匀浆中MDA值冲击后并无明显升高 (P >0 .0 5 ) ,肾组织匀浆ET 1值亦无明显升高 (P =0 .132 )。冲击后异搏定组和黄芪组SOD值降低幅度显著小于假治疗组 (P均 <0 .0 5 ) ,且黄芪组血中该低谷值提前至 2 4h。肾组织细胞学观察异搏定组损伤明显轻于假治疗组 ,而黄芪组较异搏定组更轻、恢复更快。 结论 黄芪对高能冲击波致肾损伤具有保护作用。

Objective To study the protective effects of Astragalus membranaceus on renal damage induced by ESWL in rabbits. Methods 45 rabbits were randomly divided into 3 groups:sham therapy (physiological saline) as group 1,verapamil (control) as group 2 and Astragalus membranaceus as group 3.These groups were intravenously given physiological saline (20 ml),verapamil (0.4 mg/kg) and Astragulus memebranaceus (2.4 g/kg),respectively,3 days before ESWL was applied (1500 shocks,18kV,MZ-V,made in China).Blood and homogenate ET-1,MDA,related to renal injury,and SOD,related to renal protection,and renal morphology were observed. Results After shocking,the increase of ET-1 and MDA in group 2 and group 3 was significantly lower than that in group 1(P<0.05).The ET-1 and MDA levels recovered to normal in 1 week.MDA in group 3 was not significantly elevated compared with pre-shocking(P>0.05),and ET-1 was slightly elevated (P=0.132).After shocking,SOD in groups 2 and 3 was significantly higher than that in group 1,and its trough level in group 3 earlier appeared at 24 hours after shocking.Histopathology showed that renal damage in groups 3 and 2 was much milder than that in group 1;furthermore,renal damage was even milder and its recovery was even sooner in group 3 (Astragalus membranaceus) than in group 2 (verapamil). Conclusions The results demonstrate that Astragulus membranaceus has protective effects on renal damage induced by ESWL.