干眼患者结膜上皮细胞的凋亡与炎症

The Apoptosis and Inflammation of Conjunctival Epithelium in Dry Eye Ratients

目的了解干眼患者结膜上皮细胞是否存在凋亡与炎症状态,进一步理解干眼的发病机制。方法印迹细胞法获得干眼6例22眼和正常人7例10眼的结膜上皮细胞。Annexin V-FITC/PI流式细胞技术(flow cytometric.FCM)定量检测凋亡;免疫印迹细胞化学染色检测核转录因子-kB(NF-kB)和转化生长因子-β1(TGF-β1)的表达。结果干眼患者结膜上皮细胞的凋亡指数较正常对照组明显增高(P<0.05),NF-kB和TGF-β1的表达较正常增强。凋亡指数与NF-kB和TGF-β1的表达呈正相关。结论干眼患者结膜上皮存在细胞凋亡及炎症,眼表上皮细胞的炎症可能是引起细胞凋亡的重要原因,抑制炎症是干眼的重要治疗方法。

Purpose To evaluate the apoptosis and inflammation of conjunctival epithelial cell in dry eye patients. Methods Specimens for conjunctival epithelial cells were collected by impression from 16 dry eye patients (22 eyes) and 7 normal subjects (10 eyes) . Flow cytometry was taken to measure apoptosis. Immunohistochemical staining was performed to detect nuclear factor-кB( NF-кB) and transforming growth factor-β1( TGF-β1) .Results The apoptotic index was significantly higher in conjunctival epithelium of dry eye patients than that of normal subjects (P < 0.05). The expression of NF-кB and TGF-β1 in conjunctival cytology samples was stronger in dry eye samples than that in normal subjects. The apoptotic index showed significant relationship with NF-кB( r = 0.581, P = 0.004) and TGF-β1 (γ= 0.650, P = 0.001) staining. Conclusion The apoptosis and inflammation were significantly stronger in the conjunctival epithelium of dry eye patients. The increased percentage of apoptotic cell in conjunctival epithelium may have close relationship with ocular surface inflammation.