南海西沙海域海水细菌学调查及药敏检测

Investigation of the bacteria in the seawater of Xisha in the South China Sea and their antibiotic sensitivity profile

目的了解我国南海西沙海域海面水细菌种类分布及对抗菌药物的敏感性。方法对取样海水进行细菌分离并计数,应用Phoenix-100细菌分析仪进行细菌鉴定和药敏试验。结果80份海水样本检出12种共84株细菌,其中溶藻弧菌38株(45.24%)、少动鞘氨醇单胞菌11株(13.10%)、土生丛毛单胞菌9株(10.72%)、假单胞菌属7株(8.33%)、莫拉氏菌属6株(7.14%)、西地西菌5株(5.95&),其他细菌(气单胞菌、不动杆菌各2株,成团泛菌、金氏杆菌、腐败西互菌和产吲哚萨顿菌各1株)8株,药敏结果显示,16种抗生素中,除溶藻弧菌、少动鞘氨醇单胞菌和土生丛毛单胞菌对氨曲南、哌拉西林,少动鞘氨醇单胞菌对头孢噻肟、头孢吡肟敏感率较低外,其余抗生素均呈高度敏感性。结论本次调查对了解南海西沙海域海水细菌种类分布及为海上意外伤、战伤早期防治细菌感染的抗菌药物应用有重要意义。

Objective To investigate the distribution and antibiotic sensitivity of the bacteria in the seawater of Xisha in the South China Sea. Methods The samples of the seawater in Xisha were collected for bacterial counting, identification and antibiotic sensitivity tests. Result Of the 84 bacterial strains isolated from the 80 seawater samples, 38 (45.24%) belonged to Vibrio alginolyticus, 11 (13.10%) to Sphingomonas, 9 (10.72%) to Comamonas terrigena, 7 (8.33%) to Werepseudomonas, 6 (7.14%) to Moraxella, 5 (5.95%) to Cedeceadavisae, and 8 to other bacteria including 2 Aeromona, 2 Acinetobacter, 1 Pantoea agglomerans, 1 Kingella kingae, 1 Shewanella putrefaciens and 1 Suttonella indologenes. Tests of the antibiotic sensitivity showed that all the bacteria were high sensitive to 16 kinds of the antibiotics with the exception of Vibrio alginolyticus, Sphingomonas and comamonas terrigena that were less sensitive to aztreonam or piperacillin and Sphingomonas to cefotaxime or cefepime. Conclusion The investigation will be of great significance for early-stage prevention and cure of bacterial infection acquired from the seawater.