清肝饮煎剂对对乙酰氨基酚所致肝损伤小鼠的保护

Protective Effect of Qingganying Decoction on the Murine Liver Injured by P-Acetaminophen

目的 :研究清肝饮煎剂 (qingganying ,QGY)对由对乙酰氨基酚 (AAP)所致肝损伤小鼠的保护作用。方法 :采用AAP诱发小鼠肝损伤模型 ,观察小鼠血清丙氨酸氨基转移酶 (ALT)、天门冬氨酸氨基转移酶 (AST) ,总胆红素 (SB)的变化 ,并做肝脏病理切片。结果 :各剂量QGY可降低小鼠AST活性 ,中剂量 (生药 2 8g·kg 1 )、大剂量 (生药 5 6g·kg 1 )可降低ALT活性和SB含量。病理切片观察显示 ,AAP模型组肝细胞带状坏死 ,肝细胞呈弥漫性变化 ,QGY大剂量组肝小叶结构完整、清晰 ,少量细胞点状坏死 ,少量淋巴细胞浸润 ,病变程度与AAP模型组比较减轻。结论 :QGY对AAP所致肝损伤小鼠有一定的保护作用。

Objective:To study the protective effect of qingganying (QGY) decoction on the murine liver injured by p acetaminophen (AAP). Methods:A murine model of liver injury induced by AAP served as the subject of the study. Changes in activities of alanine amiontransferase(ALT) and aspartate aminotransferanse(AST) as well as levels of total bilirubin in sera of the mice were kept under observation. Hepatic histopathology was also studied. Results:All three doses of QGY were shown to inhibit the activity of AST, medium dose (crude drug 28 g·kg 1 ) and large dose (crude drug 56 g·kg 1 ) of QGY were found to decrease the activity of ALT and lower the level of total bilirubin. Pathological sections revealed zonular necrosis and diffuse anomalies of hepatic cells in the liver of the AAP murine model untreated with QGY. In the liver of mice treated with large doses of QGY, the structure of the lobule of liver was intact and clear. Punctate necrosis of only a few hepatocytes and infiltration of a small number of lymphocytes were demonstrated. The degree of injury was relatively mild, as compared with that of the untreated model group. Conclusion:QGY decoction was shown to exert a certain protective effect on the liver injured by AAP in mice.