高效液相色谱法测定百花膏中芦丁含量

Content Determination of Rutin in Baihua Extract with HPLC

目的 :建立测定百花膏中芦丁含量的高效液相色谱法 (HPLC)。方法 :采用以控制百花膏质量的HPLC。色谱柱 :AgilentZORBAXEclipseXDB C8(4 .6mm× 15 0mm ,5 μm) ,流动相 :甲醇∶水∶冰醋酸 (2 9∶68.3∶2 .7) ,柱温 2 0℃ ,流速 :1.0mL·min 1 ,在 3 60 .0nm波长处紫外检测。结果 :该方法线性范围为 5 .5 4～ 66.48μg·mL 1 ,平均加样回收率为10 1.97% ,RSD =0 .79% (n =5 )。结论 :该法测定百花膏中芦丁的含量 ,简便、快捷、重现性好 。

Objective:To set up an HPLC method for the content determination of rutin in baihua extract. Methods:Chromatography was performed on an Agilent ZORBAX Eclipse XDB C8 column(4.6 mm×150 mm, 5 μm). The mobile phase was a mixture of methanol water glacial acetic acid (29∶68.3∶2.7) ,with a flow rate of 1.0 mL·min 1 . The column temperature was 20℃. and the detection wavelength, 360 nm. Results:The calibration curve was linear over the range of 5.54～66.48 μg·mL 1 , the average rate of recovery was 101.97%, RSD =0.79%( n =5). Conclusion:The method proved to be simple, rapid, reliable and well reproducible in the content determination of rutin in baihua extract.It may thus be used as a measure for quality control of the product.