利用光学生物传感技术筛选C5a拮抗多肽

The screening of C5a antagonist by optic biosensor technique

目的 :筛选人过敏毒素C5a的拮抗反义多肽 ,并进一步研究其分子间相互作用的动力学特性。方法 :借助于生物分子相互作用分析技术 (BIA) ,筛选可与C5a特异性结合的反义肽 ,并对其分子间的相互作用进行动力学分析。结果 :在设计合成的 4条反义肽中 ,筛选出一条反义多肽 (R4 ) ,其与C5a分子间相互作用的解离平衡常数值 (KD)o 6 6 2× 10 -6mol/L ;与其对应的功能活性区片段L2间的KD 值为 7 0 2× 10 -7。结论 :光学生物传感器是一种研究生物大分子间相互作用的理想工具 ,可用于弱亲和力分子间结合与解离的动力学分析及拮抗效应多肽的筛选。

Objective:To screen the antagonist of C5a anaphylatoxin,and further to study the dinetic characteristics of interaction between C5a and its anti-sense peptides.Methods:The anti-sense peptides were screened that can interact with C5a selectively by means of dinetic analysis,via biosensor technique.Results:There is one piece of anti-sense peptide(R4) being screened from these four synthesized peptides.The dissociation equilibrium constant(K D) between R4 and the immobilized hC5a is 6.62×10 -6 mol/L and the K D between R4 and L2 is 7.02×10 -7 . Conclusion:Based on the results obtained,it was concluded that the optic biosensor is a ideal and powerful tool to facilitate the kinetic analysis of interaction between sense peptide and its anti-sense peptide and to screen antagonist of biologic activity molecule.