HPV16 E6 DNA疫苗诱导小鼠抗宫颈癌主动免疫

Antitumor immune response to cervix carcinoma in mice induced by HPV16 E6 DNA vaccines

目的 :探讨HPV16E6DNA疫苗能否在体内诱导小鼠抗宫颈癌主动免疫应答。方法 :将HPV16E6基因转染 6 15小鼠宫颈癌U14 ,建立高表达E6的U14细胞株即E6 + U14 ;制备HPV16E6DNA疫苗 ;随后分组进行体内预防和治疗E6 + U14移植同系小鼠试验 ,设立对照组 ;观察各组小鼠肿瘤大小 ,计算抑瘤率、生存时间 ;计算淋巴和肺转移率 ;体外分别检测经HPV16E6DNA疫苗免疫后的小鼠T淋巴细胞对E6 + U14和U14的杀伤作用。结果 :HPV16E6DNA疫苗预防和治疗组小鼠各时期瘤体大小均显著低于对照组 (均P <0 .0 0 1) ,两组抑瘤率均大于70 % ;平均生存时间均大于对照组 (均P <0 .0 0 1) ;预防组淋巴转移率低于对照组 (P <0 .0 5 ) ;HPV16E6DNA疫苗免疫小鼠T淋巴细胞在不同效靶比 ,对E6 + U14的杀伤效率均明显高于对U14者 (均P <0 .0 0 1)。结论 :HPV16E6DNA疫苗能诱导机体产生针对HPV16E6阳性的宫颈癌细胞主动免疫应答及特异性细胞毒淋巴细胞 。

Objective To investigate the effect of HPV16E6 DNA vaccines on inducing the antitumor immune response to cervix carcinoma in vivo.Methods We prepared pCB/HPV16E6 DNA vaccines to prevent and treat 615 modle mice transplanted E6 +U14 in vivo respectively. Survival and tumor sizes of all mice were recorded and their lymphnodes and lung′s metastases were detected by microscopic observation. The cytotoxicity of the spleen T cells of those mice immuned with HPV E6 DNA vaccines against the E6 +U14 and U14 was detected by MTT assay.Results HPV16 E6 DNA vaccines prevented the transplanted tumor growth effectively in inbred strain mice and cured some tumor-bearing mice (P< 0.001). Pathological results showed that the number of lymphodes with metastases foci decreased in the preventing group (P<0.05). HPV16 E6 DNA vaccines induced special and higher cytotoxicity T lymphocytes against the E6 +U14 (P<0.001). Conclusion HPV16 E6 DNA vaccines can induce antitumor immune protection of the mice against E6 +U14 cells, suggesting that the vaccines may be effective in preventing and treating cervix cancer after an operation.