NMDAR1和GABA_A受体的α_1及α_3亚单位在成年猴小脑的定位分布

Distribution of NMDAR1 and subunits (α_1 and α_3) of GABA_A receptor in the cerebellar cortex of adult rhesus macaques

目的 :探讨NMDAR1和GABAA 受体的α1及α3 亚单位在成年恒河猴小脑皮质及小脑核的定位分布。方法 :用免疫组织化学染色技术及尼氏染色技术。结果 :NMDAR1免疫反应产物主要分布在Purkinje细胞胞质和分子层的树突 ,分子层的星形细胞以及颗粒细胞层的颗粒细胞呈免疫反应产物弱阳性 ,胶质细胞呈中等强度的染色 ,小脑核的神经元胞质和部分突起染色明显。GABAA 受体的α1亚单位免疫反应产物主要分布在Purkinje细胞胞质和树突 ,分子层的星形细胞和胶质细胞呈中等强度的染色 ,小脑核的神经元染色明显。GABAA 受体的α3 亚单位免疫反应产物主要分布在Purkinje细胞胞质和树突 ,胶质细胞及小脑核神经元染色明显。在Purkinje细胞层NMDAR1,GABAA 受体α1及α3 亚单位免疫阳性神经元分别占总Purkinje细胞数的 (90 .0± 2 .1) % ,(83.0± 2 .3) % ,及 (78.0± 1.8) %。结论 :NMDAR1,GABAA 受体的α1及α3 亚单位在成年恒河猴小脑具有广泛的分布。

Objective To investigate the distribution of NMDAR1 and subunits (α 1 and α 3) of GABA A receptors in the cerebellar cortex of adult rhesus macaques. Methods Immunohistochemistry and Nissl staining were used.Results ① NMDAR1 immunoreactivity was prevalent in the endoplasma of Purkinje and dendrites of molecular layer, and moderate in glia, but was very weak in the stellate cells in the molecular layer and granule cells. In addition,cerebellar nuclei were also strongly stained; ② The immunostaining pattern of subunit of GABA A α 1 receptor was similar to that of NMDAR1,but more distinctive than NMDAR1 in stellate cells; ③ Immunoreactivity of subunit (α 3) of GABA A receptor was also prevalent in the endoplasma of Purkinje cells and dendrites. The glia and the neurons of the cerebellar nucli were intensively stained. ④ The rates of Purkinje cells containing NMDAR1, and subunits of GABA A α 1 and α 3 were (90.0±2.1)%, (83.0±2.3)%, and (78.0±1.8)%, respectively. Conclusion NMDAR1 and subunits (α 1 and α 3) of GABA A receptors are widely distributed in the cerebellum of adult rhesus macaques. These receptors may play an important role in maintaining the complicated function of the cerebellar.