IL-12对乙肝病毒全S基因疫苗免疫效应的影响

Effect of interleukin-12 on HBV pan-S gene vaccine

目的 :探讨IL 12对乙型肝炎病毒全S基因疫苗的免疫效果的影响。方法 :BABL/c小鼠分为对照组、HBV全S基因表达质粒单独免疫组 (pcDNA3 PS)及pcDNA3 PS联合IL 12表达质粒免疫组 (pcDNA3 PS +IL 12 ) ,免疫后 6周测血清抗HBs,T细胞增殖反应实验及IL 2和IFN γ水平。结果 :pcDNA3 PS +IL 12组抗HBs滴度、刺激指数 (SI)、细胞因子水平均较pcDNA3 PS增高 ,差异有显著性 ;两免疫组抗HBs滴度、SI和细胞因子水平均较对照组增高 ,差异有显著性。结论 :HBV全S基因疫苗可刺激小鼠产生特异性免疫 ,IL

Objective To investigate the effect of interleukin-12 (IL-12) on immune responses induced by pcDNA3 which expresses hepatitis B virus pan-S gene.Methods BABL/c mice were immunized with pcDNA3 alone (control group), pcDNA3-PS alone, or co-immunized with pcDNA3-PS and pcDNA3-IL12.Six weeks after the immunization, sero anti-HBS antibody was examined, lymphocyte proliferation test and specific cytokine level were assayed. Results The titer of anti-HBs antibody, the stimulation index of splenocytes, and cytokine level of IL-12 and IFN-γ from the mice coimmunized with pcDNA3-PS and pcDNA3-IL12 were significantly higher than those of the mice immunized alone with pcDNA3-PS. Antibody level, SI, and cytokine level of the immunized mice were higher than those of the control group.Conclusion IL-12 expression plasmid can act as immunological adjuvants for HBV pan-S gene vaccine.