邻苯二甲酸正丁酯净化白血病细胞的作用机制

Mechanisms of dibutyl phthalate to purge leukemic cells

目的 :探讨邻苯二甲酸正丁酯通用诱导细胞凋亡来净化白血病细胞的机制。方法 :用Fura 2AM方法检测邻苯二甲酸正丁酯 (DBP)对白血病细胞内游离钙离子浓度的影响 ,用免疫组化方法检测DBP对白血病细胞c myc和Bcl 2蛋白表达的影响。结果 :DBP可引起白血病细胞内钙重新分布 ,促进细胞外钙离子内流 ,升高细胞内游离钙 ([Ca2 + ]i) ,并下调白血病细胞c myc和Bcl 2蛋白的表达。结论 :DBP可能通过提升白血病细胞内钙离子水平启动凋亡且下调c myc与Bcl 2蛋白表达。

Objective To determine the mechanism of dibutyl phthalate (DBP) to purge leukemic cells by inducing apoptosis.Methods The effects of DBP on the intracellular free Ca 2+ concentration ([Ca 2+]i) of leukemic cells were measured by Fura-2AM method. The effects of DBP on the protein expression of c-myc and bcl-2 genes of leukemic cells were measured by immunohistochemical assay.Results DBP elicited an intracellular Ca 2+ redistribution and a potent extracellular calcium influx in leukemic cells. It also down-regulated the protein expression of c-myc and bcl-2 genes. Conclusion DBP can purge leukemic cells in vitro by increasing [Ca 2+]i in cells to initiate apoptosis and down-regulate bcl-2 and c-myc proto-gene expression to promote the cell apoptosis.