大豆异黄酮对去卵巢小鼠认知功能的影响

Effect of soybean isoflavone on the cognitive function in ovariectomized mice

目的 :研究大豆异黄酮对去卵巢小鼠认知功能的影响并探讨其机制。方法 :4 5只健康雌性昆明种小鼠按体重随机均分为 5组 ,A组为对照组 (假手术组 ) ,B组为去卵巢模型组 (OVX) ,C组为OVX +大豆异黄酮低剂量组 ,D组为OVX +大豆异黄酮中剂量组 ,E组为OVX +大豆异黄酮高剂量组。试验期为 6 0d。结果 :B组小鼠到达水迷宫终点时间明显长于D组和E组 (P <0 .0 5 ) ;跳台记忆试验B组小鼠平台潜伏期短于D组 ,且错误次数高于A ,C ,D ,E组 (P <0 .0 5 )。A ,C ,D ,E组小鼠血清SOD活力、脑组织Na+ K+ ATP酶活力均高于B组 ,血清MDA水平均低于B组 (P <0 .0 5 ) ;Ca2 + Mg2 + ATP活力A ,D组高于B组 ,E组小鼠脑组织中AchE活力明显高于B ,C ,D ,组(P <0 .0 5 ) ,单胺氧化酶活力B组高于D组 (P <0 .0 5 )。结论

Objective To explore the effect of soybean isoflavone on the cognitive function in ovariectomized mice and to study the cognitive function mechanism of soybean isoflavone.Methods Forty-five Kunming female mice were randomly assigned into 5 groups: A (sham operated); B (ovariectomized, OVX); C (OVX+low dose soybean isoflavone); D (OVX+moderate dose soybean isoflavone);and E (OVX+high dose soybean isoflavone). The experiment lasted 60 days.Results Ovariectomy significantly elongated the destination time of water maze, shortened the latent time of step-down test, decreased SOD of serum and Na +K +-ATPase and Ca 2+Mg 2+-ATPase of brain, and increased malonaldehyde of serum and monoamine oxidase of brain, which could be inhibited by soybean isoflavone consumption.Conclusion The continuous oral administration of soybean isoflavone can improve the cognitive function of ovariectomized mice.