黄芪对病毒性心肌炎心脏细胞因子mRNA表达的影响

Influence on expression of cardial cytokines's mRNA of treating viral myocarditis with Astragalus membrana ceus

目的 :研究黄芪治疗病毒性心肌炎 (VMC)对心脏组织白细胞介素 2 (IL 2 )mRNA、肿瘤坏死因子 α(TNF α)mRNA及白细胞介素 10 (IL 10 )mRNA表达的影响 ,探讨其治疗VMC的免疫调节作用机制。方法 :6 0只小鼠随机分成 4组 ,其中黄芪治疗组以黄芪口服液灌胃 ,0 .2ml/次 ,2次 /d ,共 10d。采用半定量RT PCR方法检测所有存活小鼠心脏组织中细胞因子mRNA表达情况。结果 :与病毒对照组比较 ,应用黄芪治疗后 ,VMC小鼠心脏组织中IL 2mRNA与TNF αmRNA表达下降 (P <0 .0 5 ) ,而IL 10mRNA表达则升高 (P <0 .0 5 )。结论 :黄芪通过改变细胞因子在心脏组织的表达而发挥其治疗VMC的有益的免疫调节作用。

Objective:To study the influence on the expression of cytokine of IL-2 mRNA? TNF-αmRNA and IL-10 mRNA in heart tissue with viral myocarditis(VMC) after treated with Astragalus Membranaceus(AM), so its immunoregulatory mechanism of treating VMC was investigated.Method:Mice were divided into 4 groups randomly. AM was perfused to stomach twice a day with dosage of 0.2 ml each time for 10 days in the group of AM therapy.The expression of cytokines's mRNA in heart tissues was detected with semi-quantitative PCR in all sur-vived mice.Result:After treated with AM, expression of IL-2mRNA and TNF-αmRNA decreased(P< 0.05), but expression of IL-10 mRNA rised (P< 0.05)when compared with the viral control group.Conclusion:AM exerted its beneficial immunoregulatory effect in the therapy of VMC through changing expression of cytokines in heart tissue.