小鼠腹腔巨噬细胞A型清道夫受体的研究

Study on scavenger receptor A from mouse peritoneal macrophages

目的:研究A型清道夫受体在摄取氧化型低密度脂蛋白中的作用。方法:选用离体A型清道夫受体基因敲除和基因未敲除小鼠腹腔巨噬细胞作为实验对象,通过比较两组细胞对氧化型低密度脂蛋白结合和摄取的差异,来分析A型清道夫受体在摄取氧化型低密度脂蛋白中的作用。结果:基因敲除组小鼠腹腔巨噬细胞对氧化型低密度脂蛋白的摄取较基因未敲除组少35.8％,对氧化型低密度脂蛋白的降解较基因未敲除组少42％。结论:A型清道夫受体在摄取氧化型低密度脂蛋白中不占主导地位,有大约70％的氧化型低密度脂蛋白可能通过非A型清道夫受体途径被摄取。

AIM: To study the role of scavenger receptor A(SR A) in the uptake of oxidized low density lipoprotein(OxLDL) in mouse peritoneal macrophages(MPM). METHODS: Comparing the difference of the uptake of OxLDL in SR A-deficient and wild-type MPM. RESULTS: The results showed that the binding of OxLDL wasn't apparently reduced in SR A-deficient MPM. The association of OxLDL was reduced by 35.8% and degradation of OxLDL was reduced by 42% in SR A-deficient MPM compared with those in wild-type MPM. CONCLUSION: Studies showed that SR A didn't play an important role in the uptake of OxLDL in MPM. Approximately 70% of the uptake of OxLDL in macrophages is attributable to non-SR A receptor.