昆布多糖硫酸酯抑制BxPC-3细胞增殖的实验研究

Experimental study of LAMS restrain multiplication of BxPC -3 cells

目的 探讨LAMS对BxPC 3细胞增殖的抑制作用。方法 以MTT法分别测定不同浓度LAMS对BxPC 3细胞增殖的抑制作用 ,对以LAMS处理前后的BxPC 3细胞分别用TUNEL(POD)法测定凋亡细胞、免疫组化法测定其凋亡 ,Bcl 2、BaX基因蛋白含量。结果 LAMS可使BxPC 3细胞增殖抑制 ,细胞Bcl 2基因蛋白表达下降、BaX基因蛋白表达增加。结论 LAMS可有效地抑制BxPC

Objective To study the effects of LAMS for restrain multiplication of BxPC 3 cells. Methods MTT was used to determine the inhibiting action in the different concentration LAMS on BxPC 3 cells. TUNEL(POD) was used to determine the number of opoptosis cells which were treated with or without LAMS and the amount of Bcl 2?BaX was examined by using immunohistochemical staining.Results LAMS can effectively restrain the proliferation and apoptosis of cells on BxPC 3 cells and Bcl 2 expression decrease,but BaX expression increase.Conclusion LAMS can effectively restrain the proliferation on BxPC 3 cells.