摘要
在酸性条件下,硫酸庆大霉素(GEN)、硫酸妥布霉素(TOB)与甲基蓝(MB)反应,生成离子缔合物,使甲基蓝褪色,其最大褪色波长位于606nm(GEN)和610nm(TOB),表观摩尔吸光系数(ε)为1.80×104(GEN)和2.93×104(TOB)L·mol-1·cm-1;庆大霉素、妥布霉素浓度在0～1 2×10-5mol·L-1范围内遵从比耳定律。该法用于市售药物及人体尿液中庆大霉素及妥布霉素含量的测定,结果满意。
In an acetate buffer of pH 4.2, methyl blue (MB λ_(max)=604nm) reacts with the sulfate salt of gentamycin (GEN) or of tobramycin (TOB) to form complexes having their absorption maxima lying at 606nm and 610nm respectively, and leading to the color fading of MV, the absorbance of which is measured at their respective wavelength of absorption maxima. The apparent molar absorptivities of the color fading reaction in formation of complexes of GEN-MB and TOB-MB are found to be 1.80×10~4L·mol^(-1)·cm^(-1) and 2.93×10~4L·mol^(-1)·cm^(-1) respectively. Beer′s law holds in the range of 0 to 1.2×10^(-5)mol·L^(-1) for both GEN and TOB. This method has been used in the determination of contents of GEN (or TOB) in market sale drugs and in human urine.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2004年第3期166-168,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
