摘要
以大豆油和甘油为底物,在LipozymeRMIM催化下的无溶剂体系中进行甘油解制备甘二酯.详细讨论了反应时间、底物比、加酶量、反应温度和加水量等因素对甘油解反应的甘二酯得率的影响.研究结果表明:最适反应条件为反应时间6h,反应温度65℃,豆油∶甘油=2 0∶1(mol/mol),甘油加水量1%,加酶量8%,甘二酯得率为51 7%.
Soybean oil and glycerol were transesterified in solvent_free system catalyzed by Lipozyme RM IM to produce diglycerides. Effects of reaction time, substrate ratio, enzyme loading, reaction temperature and water content on the yield of diglycerides in the glycerolysis system were studied in details. The results indicated that the optimal conditions were as follows: reaction time 6 h, temperature 65 ℃, the ratio of oil and glycerol 2∶1(mol/mol), the addition of water 1%, the enzyme loading is 8%.
基金
郑州工程学院基金资助项目
