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精子DNA完整性在男性不育中的作用 被引量：13

Role of Sperm DNA Integrity in Male Infertility
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摘要 精子DNA完整性对自然受精或人工授精 ,胚胎、胎儿和婴儿的发育至关重要。研究表明 ,夫妇中丈夫精子DNA受损率超过 30 %时 ,自然受精率很低。精子DNA完整性被认为是优于传统精液参数的男性不育指标 ,已经有多种方法可用于检测 ,而且监测精子DNA完整性可用于评估欲行辅助生殖技术 ,如卵细胞胞质内单精子注射 (ICSI)治疗的夫妇。最后对精液冷冻和制备技术对精子DNA完整性的影响进行了总结。 The sperm DNA integrity is important for the success of natural or assisted fertilization, as well as normal development of the embryo, fetus and child. Some investigations have demonstrated that when the damaged spermatozoa DNA of the husband exceeds than 30%, the couple has very low potential of natural fertility. Several methods are available for the assessment of sperm DNA integrity, which is considered to be a better marker of male fertility potential than conventional semen parameters. Furthermore, monitering sperm DNA integrity may become an important part of the evaluation for couples seeking assisted reproductive techniques such as intracytoplasmic sperm injection (ICSI). Finally, the effects of cryopreservation and preparation techniques on sperm DNA integrity are summarized.
作者 朱培元 马玉苹 黄宇烽
机构地区 南京军区南京总医院生殖遗传研究室
出处 《中华男科学杂志》 CAS CSCD 2004年第3期222-226,共5页 National Journal of Andrology
关键词 精子 DNA完整性 男性不育 辅助生殖技术 sperm DNA integrity male infertility artificially reproductive technique
分类号 R698.2 [医药卫生—泌尿科学]
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参考文献25

  • 1Sakkas D, Moffatt O, Manicardi GC, et al. Nature of DNA damage in ejaculated human spermatozoa and the possible involvement of apoptosis[J]. Biol Reprod, 2002, 66(4):1061-1067.
  • 2朱培元,黄宇烽.精子细胞显微受精技术的研究进展[J].中华男科学杂志,2003,9(7):532-535. 被引量：5
  • 3Zini A, Fischer MA, Sharir S, et al. Prevalence of abnormal sperm DNA denaturation in fertile and infertile men[J]. Urology, 2002, 60(6):1069-1072.
  • 4Schmid TE, Kamischke A, Bollwein H, et al. Genetic damage in oligozoospermic patients detected by fluorescence in-situ hybridization, inverse restriction site mutation assay, sperm chromatin structure assay and the Comet assay[J]. Hum Reprod, 2003, 18(7):1474-1480.
  • 5Tomsu M, Sharma V, Miller D. Embryo quality and IVF treatment outcomes may correlate with different sperm comet assay parameters[J]. Hum Reprod, 2002, 17(7):1856-1862.
  • 6Morris ID, Ilott S, Dixon L, et al. The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development[J]. Hum Reprod, 2002, 17(4):990-998.
  • 7Benchaib M, Braun V, Lornage J, et al. Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique[J]. Hum Reprod, 2003, 18(5):1023-1028.
  • 8Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility[J]. Hum Reprod Update, 2003, 9(4):331-345.
  • 9Saleh RA, Agarwal A, Sharma RK, et al. Evaluation of nuclear DNA damage in spermatozoa from infertile men with varicocele[J]. Fertil Steril, 2003, 80(6):1431-1436.
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二级参考文献27

  • 1Levran D, Nahum H, Farhi J, et al. Poor outcome with round spermatid injection in azoospermic patients with maturation arrest [J]. Fertil Steril, 2000, 74(3) : 443-449.
  • 2Balaban B, Urman B, Isiklar A, et al. Progression to the blastocyst stage of embryos derived from testicular round spermatids [J]. Hum Reprod, 2000, 15(6) : 1377-1382.
  • 3Urman B, Alatas C, Aksoy S, et al. Transfer at the blastocyst stage of embryos derived from testicular round spermatid injection [J]. Hum Reprod, 2002, 17(3) : 741-743.
  • 4Vicdan K, Isik AZ, Delilbasi L. Development of blastocyst-stage embryos after round spermatid injection in patients with complete spermiogenesis failure [ J ]. J Assist Reprod Genet, 2001, 18(2) :78-86.
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  • 7Tesarik J, Mendoza C, Greco E. The activity ( calcium oscillator?) responsible for human oocyte activation after injection with round spermatids is associated with spermatid nuclei [ J ]. Fertil Steril, 2000, 74(6):1245-1247.
  • 8Francavilla S, Bianco MA, Cordeschi G, et al. Ultrastructural analysis of chromatin defects in testicular spermatids in azoospermic men submitted to TESE-ICSI[J]. Hum Reprod, 2001, 16(7) :1440-1448.
  • 9Carrell DT, Liu L. Altered protamine 2 expression is uncommon in donors of known fertility, but common among men with poor fertilizing capacity, and may reflect other abnormalities of spermiogenesis[J]. J Androl, 2001, 22(4) :604-610.
  • 10Kimura y, Yanagimachi R. Mouse oocytes injected with testicular spermatozoa or round spermatids can develop into normal offspring [ J ]. Development, 1995, 121 ( 8 ) : 2397-2405.

共引文献18

同被引文献112

引证文献13

二级引证文献72

中华男科学杂志
2004年 第3期

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