人宫颈癌细胞检测抗核抗体

Detection of antinuclear antibody with human cervical cancer cells

目的 :探讨用人宫颈癌细胞株Hela细胞检测抗核抗体 (ANA) ,提高ANA的阳性率。方法 :以Hela细胞为核抗原 ,采用间接免疫荧光法检测。结果 :检测120例患者血清中的ANA ,Hela细胞的检出率为54/120(45% ) ,鼠肝细胞的检出率为36/120(30% )。结论 :用人宫颈癌细胞株Hela细胞为核抗原检测ANA 。

Objective:To study howto detect the antinuclear antibody(ANA)with human cervical cancer cell strain Hela as unclear anti-gen.Methods:With Hela cell as nuclear antigen,the ANA was measured by indirect immunofluorescent assay(IIF).Results:The serum level of ANA in120patients was detected.The positive rate of Hela cells and rat hepatic cells was45%(54/120)and30%(36/120)respectively,the difference between them was significant(P<0.01).Conclusion:The results indicated that the positive rate of Hela cells was statistically higher than that of rat hepatic cells.