猫人参注射液体外抗肝癌实验研究

Experimental Study on Anti-hepatocarcinoma Effect of Maorenshen injection in vitro

目的 :观察单味猫人参注射液在体外对肝癌细胞的抑制作用。方法 :噻唑蓝还原法 (MTT法 )检测猫人参注射液对体外培养的人肝癌细胞株SMMC - 772 1、小鼠肝癌细胞株H2 2 、大鼠肝癌细胞株CBRH - 791 9的生长抑制作用。结果 :体外实验结果表明 :2 50mg ml浓度猫人参注射液对H2 2 、CBRH - 791 9、SMMC - 772 1肝癌细胞株均有抑制作用 ,抑制率分别 63 68%、41 51 %、32 92 % ,IC50 分别为 :1 0 7 70mg ml、51 9 49mg ml、667 56mg ml;在 2 4h,48h ,72h三个时相 ,对小鼠H2 2 肝癌细胞的生长抑制作用存在较明显的时效关系 ,在 72h内 1 6 88mg ml～ 2 50mg ml浓度内药物浓度与抑制作用有正相关趋势 ,其相关系数为 0 91。结论

Objective:To observe the the growth inhibition of Maorenshen injection on live cancer cell line in vitro .Methods:MTT assay was used to examine the growth inhibition of Maorenshen injection on human live cancer cell line(SMMC-7721),mice live cancer cell line(H 22 )and rat live cancer cell line(CBRH-7919).Results:Maorenshen injection had potent antitumor efficacy to three varieties of liver cancer cell lines.The inhibition rates of Maorenshen injetion on H 22 ,CBRH-7919,SMMC-7721 cell lines were 63 68%,41 51%,32 92% respectively.The IC 50 of Maorenshen injection on H 22 ,CBRH-7919,SMMC-7721 cell lines were 107 70mg/nl,519 49mg/ml,667 56mg/ml respectively.It also had time dependent relationship to H 22 during 24,48,72 hours.At the doses ranged from 16 88mg/ml to 250mg/ml,drug concentration had positive correlation to inhibition of H 22 ,R=0 91.Conclusion:Maorenshen injection had the potent antitumor activity against liver cancer cell in vitro.