猪牙皂的薄层色谱鉴别研究

Experimental Study on the Thin-layer Chromatography of Fructus Gleditsiae Abnormalis

目的 :确定猪牙皂薄层色谱鉴别的实验条件。方法 :采用 3种不同的展开剂分别进行猪牙皂薄层色谱鉴别研究。结果 :猪牙皂薄层色谱条件为 :采用硅胶G板 ,以甲苯—醋酸乙酯—甲酸 (1 4∶4∶0 5)为展开剂展开 ,1 0 %硫酸乙醇溶液为显色剂 ,在 1 0 5℃加热至斑点显色清晰。结论

Objective:To confirm the thin layer chromatography conditions of Fructus Gleditsiae Abnormalis.Method:Study on the thin layer chromatography of Fructus Gleditsiae Abnormalis with three different developers.Result:The thin layer chromatography conditions:the developer is methylbenzene ethyl acetate methanoic acid(14∶4∶0 5),color reagent is 10% sulfuric acid ethanol.Conclusion:The result is credible,the method is simple and accurate.