C_2H_2+Ar处理医用涤纶材料的细菌粘附

Surface modification of Dacron by acetylene plasma immersion ion implantation

目的 :对最常用心脏血管替代材料涤纶片作最新发展的具有全方位表面改性特征的混合等离子体浸没注入 ,以观察经处理后的涤纶片抑制细菌粘附的效果。方法 :用多功能全方位等离子体浸没及离子注入机 (PIII) ,用射频电源建立气体等离子体 ,对涤纶材料作全方位乙炔和氩气混合离子 (C2 H+ Ar)注入获取表面改性涤纶片。用金黄色葡萄球菌 ,表皮葡萄球菌 ,大肠杆菌 ,绿脓杆菌 ,白色念珠菌制取细菌悬液并作 5 12 5Ⅰ 2′ 脱氧尿嘧啶核苷 (12 5Ⅰ UDR)标记 ,再对改性涤纶材料作体外细菌动态粘附实验。结果 :表面改性涤纶材料改变了亲水性和表面能 ,降低了水分子接触角。与未改性材料相比 ,改性涤纶材料抗细菌粘附能力有较明显提高。结论 :混合离子 (C2 H2 +Ar)表面改性涤纶片有良好的抗细菌和血小板粘附能力。

Objective:The study aimed at the surface modification of cardiovascular biomaterials (Dacron) by means of Acetylene and argon (C 2H 2+Ar) plasma immersion ion implantation (PIII) and its adhesive ability with different kind of pathogenic bacterials. method: The bacterial adhesion to the modified dacron was studied quantitatively by the plate counting and Gamma-ray counting of 125 I radiolabeled 5 kinds of bacterials in vitro. result:it revealed that PIII improved water wettability,increase surface energy,decreased the water contact angle of dacron.The bacterial counting and gamma-ray counting of 125 I-radiolabeled bacteria in vitro indicated that bacterials adhesion on dacron was reduced by C 2H 2+Ar implantation at suitable energy level.Furthermore,it showed that amorphous carbon structure appears after C 2H 2 implantation.Conclusion:this suggests that the improvement of water wetability of the modified dacron might come from the destruction of original Surface chemical bond and formation of an amorphous carbonphase by PIII.It manifested that the composition change of the modified surface layer can cause the improvement of the bacterial adhesion.