转化生长因子β1与病毒性乙型肝炎患者肝细胞损伤和肝纤维化的关系

Relation of transforming growth factor-β1 to hepatocytic damage and hepatic fibrosis in patients with hepatitis B

目的本文旨在观察慢性乙型肝炎(CHB)、肝硬化(LC)患者血清转化生长因子β1(TGF-β1)与肝细胞损伤、肝脏纤维组织增生的关系。方法采用酶联免疫吸附试验(ELISA)测定52例CHB、LC患者血清TGF-β1。结果①CHB轻、中、重度患者,LC患者血清TGF-β1水平依次升高(P<0.0l或P<0.05),明显高于对照组(P<0.01)。②血清TGF-β1与Ⅲ型前胶原蛋白(PⅢP)、层粘连蛋白(LN)、透明质酸(HA)、Ⅳ型胶原(Ⅳ-C)呈正相关(P<0.01或P<0.05)。③ALT>600 U/L组、SB>125μmol/L组血清TGFβ1水平明显高于肝功能正常组和轻度受损组。(P<0.01或P<0.05)。结论血清TGF-β1的检测可用于肝纤维化程度和肝细胞受损程度的判断。

Objective To investigate the relation of transforming factor-β1 (TGF-β1 ) to hepatocytic damage and hepatic fibrosis in patients with hepatitis B. Methods The serum level of TGF-β1 was detected by ELISA in 35 patients with chronic hepatitis B (CHB) and 17 with liver cirrhosis (LC). Twenty healthy individuals were employed to serve as the control. Serum levels of collagen type Ⅳ (Ⅳ-C), laminin (LN), procollagen peptide ( PⅢ P) and hyaluronic acid (HA) were determined by radioimmunoassay. Results ① The serum level of TGF-β1 was significantly higher in the patients than in the controls (P< 0.01 ).② The serum level of TGF-β1 was positively correlated to serum levels of IV-C, LN, PⅢ P and HA (P<0.01 or 0.05). ③ The serum level of TGF-β1 was markedly higher in patients with serum ALT level > 600 U/L and those with serum TB level> 125 μmol/L than in those with normal liver function. Conclusions In patients with CHB and LC, the serum level of TGF-β1 is consistent with serum indexes of hepatic fibrosis and hepatocytic damage.