黄柏中盐酸小檗碱和盐酸巴马汀提取方法研究

Solvent extraction of berberine hydrochloride and palmatine hydrochloride in Cortex Phellodendri

目的 :建立中药黄柏中生物碱的提取方法。方法 :以盐酸小檗碱和盐酸巴马汀为指标 ,用高效液相色谱法进行分析测定 ,系统考察不同溶剂、不同提取时间、不同提取方式对盐酸小檗碱和盐酸巴马汀提取率的影响 ,并对不同来源黄柏药材中的盐酸小檗碱和盐酸巴马汀进行了测定。结果 :用盐酸 甲醇 (1∶10 )溶液超声 2 0min的提取方法可以获得较高的盐酸小檗碱和盐酸巴马汀提取率。结论 :溶剂种类和提取方式对黄柏中盐酸小檗碱和盐酸巴马汀的提取效率有较大影响。

AIM: To establish an extraction of alkaloid from Cortex Phellodendri METHODS : Taking berberine hydrochloride and palmatine hydrochloride in Cortex Phellodendri as evaluating index determined by the HPLC,the effects of different solvents,extraction time,and extraction models on extraction rate of the berberine hydrochloride and palmatine hydrochloride were systematically investigated. Berberine hydrochloride and palmatine hydrochloride in Cortex Phellodendri from different sources were also involved. RESULTS : The ultrasound extraction with hydrochloric acid-methanol (1∶100) up to 20min was more effective. CONCLUSION : The kinds of solvent and models of extraction have a great influence upon the extracting efficiency of berberine hydrochloride and plamatine hydrochloride.