HPLC测定藤茶不同采收时期及不同部位的二氢杨酶素含量

Determination of dihydromyricetin in different parts of Ampelopsis grossedentata in different seasons by HPLC

目的 :建立HPLC法对不同产地不同采收期藤茶叶、茎中的二氢杨梅素进行含量测定。方法 :采用NovapakC18柱(15 0mm× 4 .6mm ,5 μm) ,甲醇 水 磷酸 (2 7∶73∶0 .1)为流动相 ,流速为 1.0mL·min-1;紫外检测波长为 2 90nm ;柱温为 2 5°C。结果 :本法线性关系良好 ,平均加样回收率为 99.4 7% ,RSD为 1.6 8%。 5月份藤茶叶中二氢杨梅素含量最高2 7.8%～ 31.2 % ;叶中含量高于茎中 3～ 4倍。结论 :本方法操作简便 ,分离效果好 ,为藤茶的开发利用提供一定的理论依据。

AIM: To establish the determination of dihydromyricetin in stem,leaf of Ampelopsis grossedentata in different seasons from different habitat. METHODS : Novapak C_ 18 column (150mm×4.6mm,5μm) was selected as separation column at 25 °C . Methanol-water-phosphoric acid (27∶73∶0.1) was used as a mobile phase at a flow rate of 1.0mL·min -1 . The peak of dihydromyricetin was detected at UV 290nm. RESULTS : The linearity of this method was good. The average recovery was 99.47%,RSD was 1.68%. The content of dihydromyricetin in leaf of Ampelopsis grossedentata collected in May was the highest and was three ～ four times the size of that in stem. CONCLUSIONS :The method is convient with a good separating degree and is useful basis for the develoment and utilization of Ampelopsis grossedentata.