血清冻存13年后乙肝病毒表面抗原检测结果的评价

Comparison of HBsAg Test and Re-test in Sera That Has been Freezed for 13 Years

目的 :评价血清常规冻存 13年后HBsAg的检测结果。方法 :采用酶免疫测定法 (EIA)对保留血清 2 5 5份作HB sAg检测 ,并与原用单克隆抗HBsAg诊断血细胞 (McAb -RPHA)检测的结果作一致性检验 (Kappa分析 )。结果 :以EIA为标准 ,与原McAb -RPHA检测的结果比较 ,McAb -RPHA检测的假阳性率为 12 .5 0 % (1/ 8) ,假阴性率为 0 81% (2 /2 4 7)。McAb -RPHA与EIA两种方法之间 ,即同一批血清 13年前后两次检验的符合率为 98 82 % ,k =0 8175。结论 :在 - 2 0℃环境下经 13年冻存后 ,血清HBsAg检测不受影响。

Objective:To assess the results of test and retest for HBsAg in sera that kept in freezing for more than 13 years. Methods:Two hundred and fifty-five samples were retest for HBsAg by EIA,comparing with the results those were tested 13 years ago by McAb-RPHA,and assessing by Kappa statistic.Results: Based on the EIA, the false positivity rate of McAb-RPHA was 12.5% (1/8),the false negativity rate was 0.81%(2/247). The agreement rate of McAb-RPHA and EIA, i.e., the agreement rate of the HBsAg results before and after in these same samples was 98.82%, with a k value of 0.8175. Conclusion:Sera-HBsAg test result is not affected in 13 years, in the condition of -20℃.