山银花中皂苷类成分研究

Studies on the Saponins from Lonicera confusa DC.

目的 :探讨山银花药效物质基础 ,全面评价金银花类药材质量。方法 :对山银花乙醇提取物的正丁醇萃取部分进行化学成分研究 ,并利用各种化学、色谱和光谱技术鉴定其化学结构。结果 :从山银花中分离得到 7个常春藤皂苷类化合物 ,经鉴定为常春藤皂苷元 2 8 O β D 吡喃葡萄糖基 (6→ 1) O β D 吡喃葡萄糖基酯 (Ⅰ )、常春藤皂苷元 3 O α L 吡喃阿拉伯糖基 (2→ 1) O α L 吡喃鼠李糖苷 (Ⅱ )、常春藤皂苷元 3 O α L 吡喃阿拉伯糖基 (2→ 1) O α L 吡喃鼠李糖基 (3→ 1) O β D 吡喃葡萄糖苷 (Ⅲ )、常春藤皂苷元 3 O α L 吡喃阿拉伯糖基 (2→ 1) O α L 吡喃鼠李糖基 (3→ 1) O β D 吡喃葡萄糖基 (4→ 1) O β D 吡喃葡萄糖苷 (Ⅳ )、3 O α L 吡喃阿拉伯糖基 (2→ 1) O α L 吡喃鼠李糖基 常春藤皂苷元 2 8 O β D 吡喃葡萄糖基 (6→ 1) O β D 吡喃葡萄糖基酯 (Ⅴ )、3 O α L 吡喃阿拉伯糖基 (2→ 1) O α L 吡喃鼠李糖基 (3→ 1) O β D 吡喃葡萄糖基 常春藤皂苷元 2 8 O β D 吡喃葡萄糖基 (6→ 1) O β D 吡喃葡萄糖基酯 (Ⅵ )及 3 O α L 吡喃阿拉伯糖基 (2→ 1) O α L 吡喃鼠李糖基 (3→ 1) O β D 吡喃葡萄糖基 (4→ 1) O β D 吡喃葡萄糖基 常春藤皂苷元 2

AIM:To study the saponins,from the buds of Lonicera confusa DC.,and to estimate the quality of “Flos Lonicera”(Jinyinhua,in Chinese) more roundly.METHOD: The n-BuOH fraction from EtOH ext.of Lonicera confusa. were studied.Chromatography and spectral analysis were used to isolate and elucidate the constituents.RESULT: Seven triterpenoidal saponins having hederagenin as aglycones were isolated from the dried buds of L.confusa ,and identified as : hederagenin-28 -O- β- D -glucopyranosyl(6→1) -O- β- D -glucopyranosyl ester(Ⅰ),hederagenin-3 -O- α- L -arabinopyranosyl(2→1) -O- α- L -rhamnopyranosider(Ⅱ),hederagenin-3 -O- α- L -arabinopyranosyl(2→1) -O- α- L -rhamnopyranosyl-(3→1) -O- β- D -glucopyranoside (macranthoside A)(Ⅲ),hederagenin-3 -O- α- L -arabinopyranosyl(2→1) -O- α- L -rhamnopyranosyl-(3→1) -O- β- D -glucopyranosyl(4→1) -O- β- D -glucopyranoside(macranthoside B)(Ⅳ),3 -O- α- L -arabinopyranosyl-(2→1) -O- α- L -rhamnopyranosyl-hederagenin-28 -O- β- D -glucopyranosyl(6→1) -O- β- D -glucopyranosyl ester(dipsacoside B)(Ⅴ),3- O -α- L -arabinopyranosyl-(2→1) -O- α- L -rhamnopyranosyl-(3→1)- O -β- D -glucopyranosyl-hederagenin-28 -O- β- D -glucopyranosyl(6→1) -O- β- D -glucopyranosyl ester (macranthoidin A)(Ⅵ) and 3 -O- α- L -arabinopyranosyl-(2→1) -O- α- L -rhamnopyranosyl-(3→1)-β- D -glucopyranosyl-(4→1) -O- β- D -glucopyranosyl-hederagenin-28 -O- β- D -glucopyranosyl(6→1) -O- β- D -glucopyranosyl ester (macranthoidin B)(Ⅶ).CONCLUSION:Saponin I was isolated from the genus for the first time,and saponins Ⅲ and Ⅳ were first reported among natural products which have ever been isolated and reported from Lonicera.