中国南海海绵化学成分的研究

Studies on Chemical Constituents of the Marine Sponge Biemna sp. from the South China Sea

目的 :对采自中国南海的海绵Biemnasp .的化学成分进行研究 ,从中寻找有生物活性的次生代谢产物。方法 :用硅胶、凝胶LH 2 0柱层析和HPLC对海绵Biemnasp .的乙醚提取物进行分离纯化 ,并结合现代波谱技术对得到的化合物进行结构鉴定。结果 :分离得到了五个化合物 ,其结构分别鉴定为对羟基苯甲醛 (1)、3 (hydroxylacetyl) indole(2 )、4 hydroxy 5 (indole 3 yl) 5 oxo pentan 2 one(3)、3 carboxy indole(4)和 (2 4R) ergosta 7,2 2 diene 3β ,5α ,6 β triol(5 )。

AIM: To study the chemical constituents of the marine spong Biemna sp. aiming at searching for new bioactive substances. METHOD:The Et_ 2 O extract of Biemna sp. was subjected to silica gel column chromatography,sephadex LH-20 column chromatography and HPLC affording five pure compounds(1-5),whose structures were elucidated on the basis of spectroscopic analysis and by comparison with the data reported in literature. RESULT: Five known compounds were isolated and identified as p -hdroxylbenzaldehyde(1),3-(hydroxylacetyl)-indole(2),4-hydroxy-5-(indole-3-yl)-5-oxo-pentan-2-one(3),3-carboxy-indole(4) and( 24 R )-ergosta-7,22-diene-3β,5α,6β-triol(5) respectively.