ECG、EGCG对两株耐药肿瘤细胞的细胞毒增敏作用研究

The Study of Chemotherapy Sensitizing Effect of ECG and EGCG on Two Multidrug Resistant Carcinoma Cells

目的 :研究两种儿茶素成分ECG、EGCG是否对人耐药肝癌细胞BEL 74 0 4 /Adr和耐药口腔癌细胞KBV2 0 0存在细胞毒增敏作用及可能的机制。方法 :用MTT法检测药物对体外培养的细胞的毒性作用 ,用RT PCR法检测MDR1表达 ,流式细胞仪测定P gp表达及细胞内Rh 12 3含量。结果 :两种儿茶素成分在 10 0 μg·ml-1以下剂量对两株耐药肿瘤细胞的抑制率均小于 10 % ,6 0 μg·ml-1ECG或 14 μg·ml-1EGCG联合 0 8μg·ml-1ADM或 0 12 μg·ml-1VCR时MDR1下降 2 3 9%～ 38 6 % ,两种儿茶素成分与抗肿瘤药物联合应用可使P gp表达下降 ,能明显提高细胞内Rh 12 3的含量。结论 :儿茶素成分ECG、EGCG与ADM或VCR联合应用可增强耐药肿瘤细胞BEL 74 0 4 /Adr和耐药口腔癌细胞KBV2 0 0的细胞毒作用 ,其机制可能与降低MDR1 mRNA表达、下调P gp表达及抑制P gp的功能有关。

AIM:To study the chemotherapy sensitizing effect of ECG and EGCG on human multidrug resistant cells BEL-7404/Adr and KBV200 and its possible mechanism.METHOD:MTT was used to test the cytotoxicity of drugs.MDR_ 1 expression was detected by RT-PCR.FACS was used to determine the expression of P-gp and the concentration of Rh123 in carcinoma cells.RESULT:Both of the cell inhibition rate of two catechin monomers are under 10% at the dose of less than 100 μg·ml -1 .MDR_ 1 expression was reduced at 23.9%～38.6% by 60 μg·ml -1 ECG or 14 μg·ml -1 EGCG combined with 0.8 μg·ml -1 ADM or 0.12 μg·ml -1 VCR.The combination also decreased the expression of P-gp and enhanced the concentration of Rh123 in carcinoma cells.CONCLUSION:ECG or EGCG combined with chemicals respectively could enhance the cytotoxicity of chemicals on BEL-7404/Adr or KBV200.The mechanism was possibly related to decreasing the expression of MDR_ 1 -mRNA,P-gp and inhibiting the function of P-gp.