摘要
目的 分析山医群体中国地鼠E家系的遗传纯度。方法 应用经过筛选的 3 1条随机引物对中国地鼠E家系 12只个体基因组DNA进行RAPD扩增 ,计算近交个体间的相似系数。结果 所有样品的相似系数0 943 1到 0 9978之间变动 ,平均相似系数为 0 9749,聚类分析得到了这些个体的同源树资料。
ObjectiveTo analyze the genetic purity of Chinese hamsters E Genealogy inbred strain. Method31 RAPD primers were selected and used to amplify 12 sam ples of E genealogy of Chinese hamster. The amplified fragment bands were analy zed to calculate the similarity index.Result In this study ,the similarity index of all samples ranged from 94 3 1% to 99 78%, averaging at 97 49%. ConclusionChinese hamster of Shanyi colony E genealogy has reached a high degree of genetic purity.
出处
《中国实验动物学报》
CAS
CSCD
2004年第1期29-34,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
科技基础性工作专项资金资助 ( 2 0 0 2DEA1 0 0 1 0 )
