摘要
以云南拟单性木兰种子为材料,对三种DNA的提取方法(CTAB法、SDS法、高盐—低pH值法)进行了比较。结果表明:CTAB法最适于拟单性木兰种子DNA的提取,且适宜于RAPD分析,SDS法次之,高盐—低pH值法不适合。
Comparison test was carried out on 3 separation methods of DNA from Parakmeria yunnanensis seed resulted that CTAB method was the best one, then SDS, and high salinity-low pH was not recommended. The methods could be applied in identification for seed purity of Parakmeria seed.
出处
《浙江林业科技》
2004年第1期24-26,38,共4页
Journal of Zhejiang Forestry Science and Technology
