非糖尿病与糖尿患者群LMNA1908C/T基因多态性与血脂代谢紊乱的关系

Relation of LMNA1908C/ T polymorphism with dyslipidemia in non-diabetic and diabetic groups

目的:研究中国大连地区非糖尿病和糖尿患者群LMNA1908C/T基因多态性与血脂代谢紊乱的相关性。 方法:选取50例非糖尿病患者(非糖尿病组)。118例2型糖尿病患者(糖尿病组);平均年龄为(62.6±12.9)岁。测量空腹血糖、总胆固醇、三酰甘油和高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、胰岛素和C肽等。采用聚合酶链式反应-限制性内切酶长度多态性(PCR-RFLP)技术分析LMNAl098C/T基因型。 结果:LMNA1908C/T基因型分布和等位基因频率在非糖尿病和糖尿病之间没有差异。无论是非糖尿病还是糖尿病者有血脂紊乱者和无血脂紊乱者之闻差异显著。非糖尿病有血脂紊乱者的LMNAl908T/T基因型频率(51.8％)高于糖尿病患者(38.9％),LMNA190SC/T和/T/T基因型的胆固醇[(4.81±0.71),(6.32±0.81)mmol/L]、三酰甘油[(1.72±0.49),(2.22±1.09)mmol/L]水平均高于C/C型携带者[(4.00±0.82),(1.08±0.43)mmol/L],HDL-C水平[(1.21±0.21),(1.03±0.28)mmol/L]低于C/C型携带者[(1.28±0.18)mmol/L]。在糖尿病患者T/T型携带者对三酰甘油作用消失(P>0.05)。 结论:无论在非糖尿病还是糖尿病个体LMNA1908C/T多态性与血脂紊乱密切相关,尤其在高胆固醇血症者存在T等位基因剂量依赖性。

ADM: To study the relation between LMNA 1908C/T polymorphism and dyslipidemia in non-diabetic and diabetic subjects in Dalian city of China. METHODS: Fifty non-diabetic subjects (non-diabetic group) and 118 patients with type 2 diabetes were selected, with the average age of (62. 6 ± 12. 9) years old. Fasting glucose (FG), total cholesterol (TC), triglycerides (TG) and high density lipoprotein cholesterol (HDL-C), insulin and C pep-tide were measured. Genotyping distribution of LMNA1908C/T was analyzed with olymorphism chain reaction-restriction fragment length polymorphism (PCR-RFLP).RESULTS: No significant difference was found in the genotype distribtion of LMNA1908C/T and allele frequency between the non-diabetic and diabetic group, while there was significant difference between patients with or without dyslipidemia in both groups. The LMNA 1908T/T genotypic frequency of subjects with dyslipidemia in the non-diabetic group(51. 8% ) was higher than that of diabetic patients (38.9% ), levels of TC[(4. 81 ±0.71), (6. 32 ±0.81) mmol/L], TG[ (1. 72±0. 49), (2. 22 ±1.09) mmol/L] were higher in the subjects with LMNA1908C/T and /T/T than those with C/C[(4.00±0.82), (1.08±0.43) mmol/L], while HDL-C level [(1.21±0.21), (1.03±0.28) mmol/L] were lower than those with C/C [(1.28±0. 18) mmol/L]. The effect of the polymorphism on triglycerides level was lost in diabetic patients with T/T genotype. CONCLUSION: The LMNA 1908C/T polymorphism is closely related to dyslipidemia in both non-diabetes and diabetes subjects, and especially, T allelomorph dosage dependence exists in the patients with hypercholes-terolemia.