银杏黄酮甙对人巨细胞病毒感染促进动脉粥样硬化发生和发展的保护作用

Protective effect of ginkgo flavone glycoside on the improvement of the initiation and progression of atherosclerosis by human cy- tomegalovirus

目的:探讨银杏黄酮甙对人巨细胞病毒(human cytomegalovirus,HCMV)感染在促进动脉粥样硬化发生和发展中的保护作用。 方法:本实验分为4组,分别为正常对照组,银杏黄酮甙组,HCMV感染组,HCMV感染+银杏黄酮甙组。用MTT比色法观察细胞增殖情况,采用硝酸还原酶法检测银杏黄酮甙对一氧化氮含量的影响。观察银杏黄酮甙对HCMV引起的离体培养的人脐静脉内皮细胞(hunlan umbilical vein endothelial cell,HUVEC304)增殖和分泌一氧化氮的影响。 结果:银杏黄酮甙促进HUVEC304增殖较正常对照组升高,银杏黄酮甙可以剂量依赖性地增强HUVEC304 MTT光吸收值,当加入1×10^(-5)～1×10^(-2)mol/L银杏黄酮甙时MTT光吸收值较对照组分别增加14％,16％,19％和51％(t=9.67～18.72,P<0.01),一氧化氮分泌量明显增加;HCMV感染的细胞增殖显著升高,MTT光吸收值较对照组增加91％(t=7.96,P<0.05),而一氧化氮分泌量显著降低44％(t=19.46,P<0.01);银杏黄酮甙可以剂量依赖性地抑制HCMV促进的HUVEC304增殖,当加入1×10^(-5)～1×10^(-2)mol/L银杏黄酮甙时MTT光吸收值较HCMV组分别降低5％(t=0.14,P>0.05),19％(t=26.75,P<0.05),29％(t=9.68,P<0.01)和33％(t=9.30,P<0.01),促进一氧化氮的分泌量升高57％(t=6.84,P<0.01)。 结论:银杏黄酮甙可抑?

AIM: To explore the protective effect of ginkgo flavone glycoside in promoting the initiation and progression of atherosclerosis by human cytomegalovirus (HCMV) infection. METHODS: The cultured human umbilical vein endothelial cell(HUVEC 304) were divided randomly into four groups: normal control group, ginkgo flavone glycoside group, HCMV group and ginkgo flavone glycoside + HCMV group. The cells proliferation was assayed by means of MTT chromatometry, and the concentration of NO was measured by the nitrate reductase method. The effect of ginkgo flavone glycoside on HCMV-induced proliferation of HUVEC304 cultured in vitro and secretion of NO were observed. RESULTS: Ginkgo flavone glycoside(1× 10-5 - 1 × 10-2 mol/L) enhanced more proliferation of HUVEC304 than that in the control group. It enhanced the OD values of MTT dose-dependently, which were increased by 14% , 16%, 19% and 51% respectivety (t = 9. 67-18. 72, P <0. 01), NO secretion increased obviously, HCMV infected cell proliferation increased significantly, and the OD values of MTT increased by 91% compared with the control group( 1 = 7. 96, P < 0. 05), while the NO secretion reduced by 44% obviously( t = 19. 46, P < 0. 01) . Cell proliferation was significantly inhibited by ginkgo flavone glycoside (1 × 10-5 - 1× 10-2 mol/L) dose-dependently, OD valuess of MTT reduced by 5% (t = 0. 14, P > 0.05), 19% (t=26.75, P <0.05), 29% (t=9.68, P <0.01) and 33% (t = 9. 30, P < 0. 01) compared with those in the control group, respectively, and NO secretion was increased by 57% (t =6. 84, P <0. 01). CONCLUSION: Ginkgo flavone glycoside has an inhibitive effect on HCMV-induced excessive proliferation and the depressive secretion of NO, and may have a certain effect on the prevention and cure of atherosclerosis.